Nanoscale live-cell imaging using hopping probe ion conductance microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19252505.
- Also identified by DOI 10.1038/nmeth.1306 and PMC identifier 2702483.
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Abstract
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.
Medical subject headings
- Cells, Cultured
- Microscopy, Scanning Probe
- Nanotechnology