A novel hydrophilic poly(lactide-co-glycolide)/lecithin hybrid microspheres sintered scaffold for bone repair.

Abstract

Novel 3-D porous scaffolds made of sintered poly(lacide-co-glycolide) (PLGA)/lecithin hybrid microspheres (PLGA/Lec-SMS) were developed and investigated. The addition of lecithin in PLGA bulk successfully managed the desired hydrophilic modification without sacrificing bulk properties. The outcomes were verified with infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and contact angle analyses. Specifically, this model of scaffold gained significant improvement in mechanical (mainly compressive) strength upon an optimization of lecithin fractions aligning with sintering conditions. Given a perspective of bone tissue engineering use, human fetal osteoblasts were seeded into a series of these PLGA/Lec-SMS scaffolds upon which key parameters of cytocompatibility and osteoconductivity (including cell viability, alkaline phosphatase activity, calcium secretion, and osteogenic genes expression) were assessed. Osteoblasts seeded on PLGA scaffolds with 5 wt % lecithin demonstrated high cell viability and alkaline phosphatase activity. Moreover, elevated lecithin also enhanced the expression of type I collagen. Taken together, these results suggest PLGA/Lec-SMS are promising scaffolds for bone repair.