An ultramarine fluorescent protein with increased photostability and pH insensitivity.
basic_science · Level V
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- Record sourced from PubMed, PMID 19349978.
- Also identified by DOI 10.1038/nmeth.1317.
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Abstract
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells.
Medical subject headings
- Luminescent Proteins