Single molecule-sensitive probes for imaging RNA in live cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19349979.
- Also identified by DOI 10.1038/nmeth.1316 and PMC identifier 4297622.
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Abstract
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
Medical subject headings
- Molecular Probe Techniques
- RNA