Controlling the selection stringency of phage display using a microfluidic device.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19350081.
- Also identified by DOI 10.1039/b820985e.
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Abstract
We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.
Medical subject headings
- Microfluidic Analytical Techniques
- Peptide Library