Photoconversion in orange and red fluorescent proteins.

Kremers, Gert-Jan; Hazelwood, Kristin L; Murphy, Christopher S; Davidson, Michael W; Piston, David W · Nat Methods · 2009

basic_science · Level V

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Abstract

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

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