Universal sample preparation method for proteome analysis.
basic_science · Level V
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- Record sourced from PubMed, PMID 19377485.
- Also identified by DOI 10.1038/nmeth.1322.
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Abstract
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
Medical subject headings
- Analytic Sample Preparation Methods
- Mass Spectrometry
- Proteome
- Proteomics