Transcriptional network of p63 in human keratinocytes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19390658.
- Also identified by DOI 10.1371/journal.pone.0005008 and PMC identifier 2672041.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin.
Medical subject headings
- Gene Expression Regulation
- Gene Regulatory Networks
- Keratinocytes
- Skin
- Trans-Activators
- Tumor Suppressor Proteins