Extracellular matrix gene expression during wound healing of the injured rat vocal fold.

Abstract

To measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3 during the inflammatory, proliferative, and remodeling phases of rat vocal fold injury. Prospective, animal model. Vocal folds were injured in 30 rats. Injured specimens were harvested on postinjury days 1, 3, 7, 14, 28, and 56. Five uninjured rats were used for polymerase chain reaction (PCR) control. Real-time PCR was used to measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3. Compared with control, expression of procollagen-I and -III were significantly decreased on postinjury days 1 and 56; decorin expression was significantly decreased on postinjury days 1, 3, 7, and 56; HAS-1 expression was significantly decreased on postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56. Results revealed time-dependent alterations in the expression of genes coding procollagen-I and -III, decorin, and HAS-1, -2, and -3. Knowledge of the temporal regulation of these genes and underlying histology will be used in future studies to investigate molecular approaches for manipulation of vocal fold injury.