Seeding-induced self-assembling protein nanowires dramatically increase the sensitivity of immunoassays.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19402649.
- Also identified by DOI 10.1021/nl9003464.
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Abstract
Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia pestis F1 antigen.
Medical subject headings
- Enzyme-Linked Immunosorbent Assay
- Nanowires