Automated unrestricted multigene recombineering for multiprotein complex production.
basic_science · Level V
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- Record sourced from PubMed, PMID 19412171.
- Also identified by DOI 10.1038/nmeth.1326.
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Abstract
Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.
Medical subject headings
- Escherichia coli
- Multigene Family
- Protein Engineering
- Recombinant Proteins