TU-tagging: cell type-specific RNA isolation from intact complex tissues.
basic_science · Level V
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- Record sourced from PubMed, PMID 19430475.
- Also identified by DOI 10.1038/nmeth.1329 and PMC identifier 2783170.
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Abstract
We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
Medical subject headings
- Drosophila melanogaster
- Pentosyltransferases
- RNA
- Thiouracil
- Tissue Extracts