Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics.

Grinberg, Olga; Gedanken, Aharon; Patra, Chitta Ranjan; Patra, Sujata; Mukherjee, Priyabrata; Mukhopadhyay, Debabrata · Acta Biomater · 2009

basic_science · Level V

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Abstract

This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [(3)H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.

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