Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19478942.
- Also identified by DOI 10.1371/journal.pone.0005730 and PMC identifier 2684634.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
Medical subject headings
- Biological Assay
- Prions