Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19483679.
- Also identified by DOI 10.1038/nbt.1545 and PMC identifier 2891235.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry. When combined with iTRAQ labeling, SNO-RAC revealed that intracellular proteins may undergo rapid denitrosylation on a global scale. This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation.
Medical subject headings
- Proteome
- Proteomics
- S-Nitrosothiols
- Sulfhydryl Compounds