Protein interaction platforms: visualization of interacting proteins in yeast.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19483691.
- Also identified by DOI 10.1038/nmeth.1337 and PMC identifier 2819136.
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Abstract
Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.
Medical subject headings
- Protein Interaction Mapping
- Saccharomyces cerevisiae Proteins