Stability analysis of recombinant human TFF2 and its therapeutic effect on burn-induced gastric injury in mice.

Sun, Yong; Wu, Wei; Zhang, Yong; Lv, Shangjun; Wang, Lin; Wang, Shiliang; Peng, Xi · Burns · 2009

basic_science · Level V

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Abstract

The trefoil factor family 2 (TFF2), a member of the trefoil factor family, plays a critical role in maintaining homeostasis throughout the gastrointestinal tract. In the present study, we expressed recombinant human TFF2 by Escherichia coli expression system with the purity above 95%. In an in vitro simulated gastrointestinal environment, rhTFF2 was shown to exhibit resistance to proteases, heat, acid and alkali. In addition, in mouse burn-induced gastric injury models, rhTFF2 was demonstrated to accelerate the healing of gastric mucosal lesions. This study provides a new way to treat burn-induced gastric injury.

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