Parallax: high accuracy three-dimensional single molecule tracking using split images.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19496608.
- Also identified by DOI 10.1021/nl901129j and PMC identifier 2728077.
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Abstract
Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.
Medical subject headings
- Imaging, Three-Dimensional