Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 19513899.
- Also identified by DOI 10.1080/14653240902887259.
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Abstract
A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
Medical subject headings
- Cell Separation
- Fetal Blood
- Mesenchymal Stem Cells
- Stromal Cells