Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19525957.
- Also identified by DOI 10.1038/nmeth.1342 and PMC identifier 3555078.
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Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
Medical subject headings
- Agouti Signaling Protein
- Embryonic Stem Cells
- Mice, Inbred C57BL