Quantitative analysis of gene expression in a single cell by qPCR.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19525960.
- Also identified by DOI 10.1038/nmeth.1338.
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Abstract
We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.
Medical subject headings
- Gene Expression
- Polymerase Chain Reaction