Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19543286.
- Also identified by DOI 10.1038/nmeth.1344 and PMC identifier 2731062.
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Abstract
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.
Medical subject headings
- Immunologic Techniques
- T-Lymphocyte Subsets