Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications.

McNamara, Laura E; Dalby, Matthew J; Riehle, Mathis O; Burchmore, Richard · J R Soc Interface · 2010

review · Level V

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Abstract

Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE-both minimal and saturation labelling-to biomaterials research, considering the challenges and rewards of this approach.

Medical subject headings