Conditional and reversible disruption of essential herpesvirus proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19578384.
- Also identified by DOI 10.1038/nmeth.1346.
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Abstract
Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Medical subject headings
- Genes, Essential
- Genes, Viral
- Herpesviridae
- Viral Proteins