Variation in the safety of induced pluripotent stem cell lines.

Miura, Kyoko; Okada, Yohei; Aoi, Takashi; Okada, Aki; Takahashi, Kazutoshi; Okita, Keisuke; Nakagawa, Masato; Koyanagi, Michiyo et al. · Nat Biotechnol · 2009

basic_science · Level V

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Abstract

We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.

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