Reduction of canine plasminogen leads to an expanded molecule which precipitates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19593387.
- Also identified by DOI 10.1371/journal.pone.0006196 and PMC identifier 2703797.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Canine plasminogen is made up of seven domains. In each domain there are several cysteines that are linked by disulfide bonds. Reduction of a limited number of the cystines destabilizes the protein such that it precipitates. The bond or bonds that are broken provide about 14 kcal of stabilization energy. Circular dichroism and dynamic light scattering indicate that there is probably an intermediate that is formed prior to precipitation and that the intermediate is somewhat larger than the compact form of plasminogen.
Medical subject headings
- Plasminogen