Differences between CusA and AcrB crystallisation highlighted by protein flexibility.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19593437.
- Also identified by DOI 10.1371/journal.pone.0006214 and PMC identifier 2704858.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
BACKGROUND: Until very recently, AcrB was the only Resistance Nodulation and cell Division transporter for which the structure has been elucidated. Towards a general understanding of this protein family, CusA and AcrB were compared. METHODOLOGY/PRINCIPAL FINDINGS: In dodecylmaltoside, AcrB crystallised in many different conditions, while CusA does not. This could be due to the difference in dynamic between these proteins as judged from limited proteolysis assays. Addition of various compounds, in particular heavy metal cations, stabilises CusA. CONCLUSION/SIGNIFICANCE: This approach could constitute a first step towards CusA crystallisation.
Medical subject headings
- Escherichia coli Proteins
- ATP-Binding Cassette, Sub-Family C Proteins