Dereplication and de novo sequencing of nonribosomal peptides.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19597502.
- Also identified by DOI 10.1038/nmeth.1350 and PMC identifier 2754211.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but nonidentical NRPs, substantially reducing dereplication efforts.
Medical subject headings
- Databases, Protein
- Peptide Biosynthesis, Nucleic Acid-Independent
- Peptides, Cyclic
- Sequence Analysis, Protein