Pattern of DAP12 expression in leukocytes from both healthy and systemic lupus erythematosus patients.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19606219.
- Also identified by DOI 10.1371/journal.pone.0006264 and PMC identifier 2707004.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DAP12 is an ITAM-bearing transmembrane adaptor originally identified on the surface of Natural Killer cells. A broad expression among other immune cells was later found in myeloid and lymphoid cells. However, data on DAP12 expression pattern rely only on immunoblot and microarray analysis. Here, we describe the generation and the characterization of an anti-DAP12 monoclonal antibody. Using this novel reagent, we show that DAP12 expression is restricted to innate immune cells in basal condition. Since a decreased expression of DAP12 has been suggested in NK cells of systemic lupus erythematosus patients, we have further investigated the NK cell receptor repertoire and leukocyte expression of DAP12 in these patients and no major changes were detectable when compared to controls.
Medical subject headings
- Adaptor Proteins, Signal Transducing
- Leukocytes
- Lupus Erythematosus, Systemic
- Membrane Proteins