Microfluidic enrichment of a target cell type from a heterogenous suspension by adhesion-based negative selection.

Green, James V; Murthy, Shashi K · Lab Chip · 2009

basic_science · Level V

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Abstract

The enrichment or isolation of a selected type of cells in a heterogeneous suspension is challenging when the surface markers of these cells are not completely known. Here, we present a 3-stage arrangement of peptide-coated microfluidic channels that can recover a small number of adipose-derived stem cells (ADSCs) from a heterogenous suspension by negative selection depletion of the non-target cell types.

Medical subject headings