Virtual terminator nucleotides for next-generation DNA sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19620973.
- Also identified by DOI 10.1038/nmeth.1354 and PMC identifier 2719685.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
Medical subject headings
- Chromosomes, Artificial, Bacterial
- DNA
- Nucleotides
- Sequence Analysis, DNA