LIF-free embryonic stem cell culture in simulated microgravity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19626124.
- Also identified by DOI 10.1371/journal.pone.0006343 and PMC identifier 2710515.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
BACKGROUND: Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required. METHODOLOGY/PRINCIPAL FINDINGS: We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF. CONCLUSIONS/SIGNIFICANCE: Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
Medical subject headings
- Embryonic Stem Cells
- Leukemia Inhibitory Factor
- Weightlessness