G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.
basic_science · Level V
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- Record sourced from PubMed, PMID 19633663.
- Also identified by DOI 10.1038/nmeth.1356 and PMC identifier 2754220.
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Abstract
We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
Medical subject headings
- Cell Lineage
- DNA Nucleotidyltransferases
- DNA-Binding Proteins
- Drosophila Proteins
- Drosophila melanogaster
- Genetic Techniques
- Saccharomyces cerevisiae Proteins
- Transcription Factors