The twin spot generator for differential Drosophila lineage analysis.
basic_science · Level V
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- Record sourced from PubMed, PMID 19633664.
- Also identified by DOI 10.1038/nmeth.1349 and PMC identifier 2720837.
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Abstract
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
Medical subject headings
- Cell Lineage
- Drosophila melanogaster
- Genomics
- Mitosis
- Recombination, Genetic