An integrated microfluidic device for large-scale in situ click chemistry screening.

Wang, Yanju; Lin, Wei-Yu; Liu, Kan; Lin, Rachel J; Selke, Matthias; Kolb, Hartmuth C; Zhang, Nangang; Zhao, Xing-Zhong et al. · Lab Chip · 2009

basic_science · Level V

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Abstract

An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

Medical subject headings