Transport of live yeast and zebrafish embryo on a droplet digital microfluidic platform.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19636473.
- Also identified by DOI 10.1039/b906257b.
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Abstract
We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
Medical subject headings
- Microfluidic Analytical Techniques
- Preservation, Biological
- Saccharomyces cerevisiae
- Zebrafish