The effect of electrical stimulation on the differentiation of hESCs adhered onto fibronectin-coated gold nanoparticles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19651437.
- Also identified by DOI 10.1016/j.biomaterials.2009.07.026.
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Abstract
To encourage stem cell differentiation, gold nanoparticles (20 nm) were used to deliver electrical stimulation to human embryonic stem cells (hESCs) in vitro. Nano-structured gold nanoparticles were designed by coating the surface of culture dishes with gold nanoparticles using a layer-by-layer (LBL) system. In this method, gold nanoparticles were continuously coated onto dishes by SEM analysis. Evaluation of gene modified hESCs that were subsequently attached onto fibronectin-coated gold nanoparticles revealed that the un-differentiation marker, Oct-4, was no longer present following electrical stimulation. In addition, the osteogenic markers of collagen type I and Cbfa1 increased in response to electrical stimulation, while those of hESCs were not observed without electrical stimulation.
Medical subject headings
- Coated Materials, Biocompatible
- Electric Stimulation
- Embryonic Stem Cells
- Fibronectins
- Gold
- Nanoparticles
- Osteoblasts