Accurate determination of microbial diversity from 454 pyrosequencing data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19668203.
- Also identified by DOI 10.1038/nmeth.1361.
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Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.
Medical subject headings
- Bacteria
- Genetic Variation
- Sequence Analysis, DNA