Digital transcriptome profiling using selective hexamer priming for cDNA synthesis.
basic_science · Level V
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- Record sourced from PubMed, PMID 19668204.
- Also identified by DOI 10.1038/nmeth.1360.
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Abstract
We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 microg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing.
Medical subject headings
- Brain
- DNA, Complementary
- Gene Expression Profiling
- Gene Library