Crystallographic ab initio protein structure solution below atomic resolution.
basic_science · Level V
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- Record sourced from PubMed, PMID 19684596.
- Also identified by DOI 10.1038/nmeth.1365.
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Abstract
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A.
Medical subject headings
- Proteins