TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19706440.
- Also identified by DOI 10.1073/pnas.0900527106 and PMC identifier 2736405.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR)4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.
Medical subject headings
- Epithelial Cells
- Escherichia coli
- Exocytosis
- Toll-Like Receptor 4
- Urinary Bladder