Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19718032.
- Also identified by DOI 10.1038/nmeth.1368 and PMC identifier 6101214.
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Abstract
We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found that cytosolic Zn(2+) was buffered at 0.4 nM in pancreatic beta cells, and we found substantially higher Zn(2+) concentrations in insulin-containing secretory vesicles.
Medical subject headings
- Biological Assay
- Fluorescence Resonance Energy Transfer
- Homeostasis
- Molecular Probe Techniques
- Protein Engineering
- Recombinant Proteins
- Zinc