Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19718033.
- Also identified by DOI 10.1038/nmeth.1367 and PMC identifier 2755602.
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Abstract
Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.
Medical subject headings
- Electron Microscope Tomography
- Erythrocytes
- Image Enhancement
- Imaging, Three-Dimensional
- Nanotechnology
- Plasmodium falciparum