Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19733541.
- Also identified by DOI 10.1016/j.stem.2009.06.001 and PMC identifier 4592285.
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Abstract
Mechanisms governing muscle satellite cell withdrawal from cell cycle to enter into quiescence remain poorly understood. We studied the role of angiopoietin 1 (Ang1) and its receptor Tie-2 in the regulation of myogenic precursor cell (mpc) fate. In human and mouse, Tie-2 was preferentially expressed by quiescent satellite cells in vivo and reserve cells (RCs) in vitro. Ang1/Tie-2 signaling, through ERK1/2 pathway, decreased mpc proliferation and differentiation, increased the number of cells in G0, increased expression of RC-associated markers (p130, Pax7, Myf-5, M-cadherin), and downregulated expression of differentiation-associated markers. Silencing Tie-2 had opposite effects. Cells located in the satellite cell neighborhood (smooth muscle cells, fibroblasts) upregulated RC-associated markers by secreting Ang1 in vitro. In vivo, Tie-2 blockade and Ang1 overexpression increased the number of cycling and quiescent satellite cells, respectively. We propose that Ang1/Tie-2 signaling regulates mpc self-renewal by controlling the return to quiescence of a subset of satellite cells.
Medical subject headings
- Angiopoietin-1
- Angiopoietin-1/metabolism
- Angiopoietin-1/pharmacology
- Animals
- Apoptosis
- Apoptosis/drug effects
- Autocrine Communication
- Autocrine Communication/drug effects
- Cell Count
- Cell Proliferation
- Cell Proliferation/drug effects
- Cells, Cultured
- Fibroblasts
- Fibroblasts/drug effects
- Fibroblasts/metabolism
- Gene Silencing
- Gene Silencing/drug effects
- Humans
- Mice
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 1/metabolism
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase 3/metabolism
- Muscle Development
- Muscle Development/drug effects
- Myocytes, Smooth Muscle
- Myocytes, Smooth Muscle/drug effects
- Myocytes, Smooth Muscle/metabolism
- PAX7 Transcription Factor
- PAX7 Transcription Factor/metabolism
- Paracrine Communication
- Paracrine Communication/drug effects
- Protein Binding
- Protein Binding/drug effects
- Receptor, TIE-2
- Receptor, TIE-2/metabolism
- Satellite Cells, Skeletal Muscle
- Satellite Cells, Skeletal Muscle/cytology
- Satellite Cells, Skeletal Muscle/drug effects
- Satellite Cells, Skeletal Muscle/enzymology
- Satellite Cells, Skeletal Muscle/metabolism
- Signal Transduction
- Signal Transduction/drug effects
- Stem Cells
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/metabolism