Clear differences in levels of a formaldehyde-DNA adduct in leukocytes of smokers and nonsmokers.
cross_sectional · Level IV
Where this comes from
- Record sourced from PubMed, PMID 19738046.
- Also identified by DOI 10.1158/0008-5472.CAN-09-1571 and PMC identifier 2745488.
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Abstract
Formaldehyde is considered carcinogenic to humans by the IARC, but there are no previous reports of formaldehyde-DNA adducts in humans. In this study, we used liquid chromatography-electrospray ionization-tandem mass spectrometry to quantify the formaldehyde-DNA adduct N(6)-hydroxymethyldeoxyadenosine (N(6)-HOMe-dAdo) in leukocyte DNA samples from 32 smokers of >or=10 cigarettes per day and 30 nonsmokers. Clear peaks coeluting with the internal standard in two different systems were seen in samples from smokers but rarely in nonsmokers. N(6)-HOMe-dAdo was detected in 29 of 32 smoker samples (mean +/- SD, 179 +/- 205 fmol/micromol dAdo). In contrast, it was detected in only 7 of 30 nonsmoker samples (15.5 +/- 33.8 fmol/micromol dAdo; P < 0.001). The results of this study show remarkable differences between smokers and nonsmokers in levels of a leukocyte formaldehyde-DNA adduct, suggesting a potentially important and previously unrecognized role for formaldehyde as a cause of cancer induced by cigarette smoking.
Medical subject headings
- DNA Adducts
- Formaldehyde
- Leukocytes
- Smoking