Haplotype of multiple polymorphisms resolved by enzymatic amplification of single DNA molecules.
basic_science · Level V
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- Record sourced from PubMed, PMID 1974719.
- Also identified by PMC identifier 54520.
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Abstract
We have developed a reliable method for the direct resolution of haplotypes or linkage phase from individuals who are multiply heterozygous in a given genomic region. The method is based on single-molecule dilution (SMD) of genomic template and amplification via biphasic polymerase chain reaction (booster PCR). We have verified the feasibility of the SMD method for a highly polymorphic region within the beta-globin cluster by analysis of triply heterozygous individuals of known haplotype. This approach should be useful in many studies in population or evolutionary genetics and in a variety of clinical settings.
Medical subject headings
- DNA
- Haplotypes
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length