Inhibition of invasion and migration of salivary gland adenocarcinoma cells by 5'-nitro-indirubinoxime (5'-NIO).

Abstract

5'-Nitro-indirubinoxime (5'-NIO) is a derivative of the bis-indole indirubin that exhibits anticancer activities. The present study investigated the anti-invasive action of 5'-NIO in salivary gland ductal adenocarcinoma, SGT cells. The wound-scratch, migration, and invasion assays were applied to determine the effect of 5'-NIO on the migration capacity and invasiveness of SGT cells. Reverse-transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to evaluate the impacts of 5'-NIO on the expression of beta1 integrin and matrix metalloproteinases MMP-2 (gelatinase-A) and MMP-9 (gelatinase-B). The viability of SGT cells was decreased by 5'-NIO in a dose-dependent manner, but not significant at the concentrations of 0.5 and 1 microM. Under the concentrations showing little cytotoxic effect, 5'-NIO exhibited a dose-dependent inhibitory effect on the invasion and migration of SGT cells. Furthermore, 5'-NIO suppressed the mRNA and protein expression of beta1 integrin and MMP-2 and MMP-9. Taken together, these results suggest that 5'-NIO, even at low concentrations, may effectively inhibit the invasion and migration of SGT cells by suppressing beta1 integrin-mediated signaling.