Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19836831.
- Also identified by DOI 10.1016/j.biomaterials.2009.09.098 and PMC identifier 2788109.
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Abstract
We describe a method of using a 3D collagen gel scaffold applied at the air-liquid interface to culture dissociated primary tracheal-bronchial ciliated cells into a ciliated epithelial tissue structure (CETS). This 3D collagen gel culture system enables the induction of ciliogenesis and continuously provides support, maintenance, development, differentiation and propagation for the growth of cilia into the CETS. The CETS developed by this system resembles the ciliary metachronal motility and morphological, histological and physiopharmacological characteristics of cells found in native and in vivo ciliated epithelia. The CETS can be sustained for months with a straightforward and simple maintenance protocol. The integrity of the functional ciliary activity of this CETS enables the evaluation of long-term effects of many pulmonary drug candidates without using animals.
Medical subject headings
- Biocompatible Materials
- Cilia
- Collagen
- Respiratory Mucosa
- Tissue Culture Techniques