Nanocarrier cross-linking density and pH sensitivity regulate intracellular gene transfer.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19842673.
- Also identified by DOI 10.1021/nl902789s.
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Abstract
Treatment of diseases on the molecular level by genetic material is limited by effective delivery mechanisms. We focused on the synthesis of a pH-sensitive gene delivery vehicle based on dimethylaminoethyl methacrylate (DMAEMA) with tunable swelling, cross-linking density, and DNA release kinetics within the endosomal pH range. Our strategy, which utilized a single step for DNA encapsulation, enhanced gene transfection efficiency and reduced cytotoxicity relative to polyethyleneimine (PEI) and poly-L-lysine (PLL).
Medical subject headings
- DNA
- Drug Carriers
- Methacrylates
- Nanostructures
- Transfection