A guanidinylated bioreducible polymer with high nuclear localization ability for gene delivery systems.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19854504.
- Also identified by DOI 10.1016/j.biomaterials.2009.10.034 and PMC identifier 2813999.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Guanidinylated bioreducible polymer (GBP) was developed for gene delivery systems utilizing cellular penetrating ability of guanidine groups. GBP could retard pDNA from a weight ratio of 5 completely in agarose gel electrophoresis but pDNA was released from GBP polyplexes even at a weight ratio of 20 in reducing condition (2.5mm DTT) due to their biodegradation. GBP also could construct 200 nm-sized and positively charged (approximately 30 mV) polyplex nanoparticles with pDNA. The cytotoxicity of GBP was found to be minimal and GBP showed about 8 folds improved transfection efficiency than a scaffold polymer, poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and even higher transfection efficiency than PEI25k in mammalian cell lines. Its high cellular uptake efficiency (96.1%) and strong nuclear localization ability for pDNA delivery due to the structural advantage of bioreducible polymer and guanidine groups were also identified, suggesting GBP is a promising candidate for efficient gene delivery systems.
Medical subject headings
- Biocompatible Materials
- Cell Nucleus
- Gene Transfer Techniques
- Guanidine
- Polymers